CHINESE JOURNAL OF ENERGETIC MATERIALS
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一株高效降解苯胺菌Q6的分离鉴定及其降解特性
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中北大学环境与安全工程学院, 山西 太原 030051

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山西省重点研发计划项目(201903D321083 );中央引导地方科技发展资金项目(YDZX 20201400001443)


Isolation and Identification of a Highly Efficient Strain of Aniline Degrading Bacterium Q6 and Degradation Characteristics
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School of Environment and Safety Engineering, Central North University, Taiyuan 030051, China

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    摘要:

    火炸药在生产和加工中会产生苯胺废水,对环境造成较大的污染。为苯胺降解提供高效的菌种资源,从化工废水处理厂好氧曝气池活性污泥中分离出一株以苯胺为唯一碳源和能源生长的高效降解菌Q6,通过形态观察、生理生化特征和16S rDNA基因序列系统发育分析,初步鉴定菌株属于食酸菌属(Acidovorax sp.)。研究了接种量、温度、pH值、外加碳氮源对菌株Q6降解苯胺的影响,同时拟合了不同初始浓度苯胺的降解动力学过程和菌株Q6生长动力学过程。实验结果表明,在温度23~37 ℃和pH 5~8的条件下,菌株Q6均能高效降解苯胺,且氯化铵可作为最优共代谢氮源促进菌株对苯胺的降解。最适条件33 ℃、pH 7和接种量10%,该菌株对不同初始浓度(200~3000 mg·L-1)苯胺的降解率均可达95.0%以上,降解苯胺过程呈现一级和零级动力学关系。Q6生长过程符合Haldane方程,其最大比生长速率μmax为0.130 h-1,半饱和常数Ks为190 mg·L-1,抑制常数Ki为8497 mg·L-1。研究表明,菌株Q6在处理含高浓度苯胺工业废水方面具有独特潜力。

    Abstract:

    The production and processing of pyrotechnics produce aniline wastewater, which causes a great pollution to the environment. To provide an efficient strain resource for aniline degradation, a strain Q6 with aniline as the only carbon source and energy growth was isolated from the activated sludge in the aerobic aeration tank of the chemical wastewater treatment plant. Through morphological observation, physiological and biochemical characteristics and 16S rDNA gene sequence phylogeny analysis, the strain was identified as Acidovorax sp. The effects of inoculum amount, temperature, pH value and additional carbon and nitrogen sources on aniline degradation by strain Q6 were studied. Meanwhile, the degradation kinetics of aniline at different initial concentrations and the growth kinetics of strain Q6 were fitted. The results showed that under the conditions of temperature 23-37 ℃ and pH 5-8, strain Q6 could degrade aniline efficiently, and ammonium chloride was the best co-metabolizing nitrogen source to promote the degradation of aniline.The optimum conditions of 33 ℃, pH 7 and inoculation amount of 10%, the degradation rate of aniline at different initial concentrations (200-3000 mg·L-1) was above 95.0%, and the degradation process of aniline showed first-order and zero-order kinetics. The growth process of Q6 conformed to the Haldane equation with a maximum specific growth rate μmax of 0.130 h-1, a half-satiation constant Ks of 190 mg·L-1 and the inhibition constant Ki of 8497 mg·L-1. The results show that strain Q6 has unique potential in the treating industrial wastewater containing high concentration of aniline.

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引用本文

胡锦俊,白红娟,宋雨,等.一株高效降解苯胺菌Q6的分离鉴定及其降解特性[J].含能材料, 2022, 30(10):1004-1012. DOI:10.11943/CJEM2022168.
HU Jin-jun, BAI Hong-juan, SONG Yu, et al. Isolation and Identification of a Highly Efficient Strain of Aniline Degrading Bacterium Q6 and Degradation Characteristics[J]. Chinese Journal of Energetic Materials, 2022, 30(10):1004-1012. DOI:10.11943/CJEM2022168.

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  • 收稿日期: 2022-06-24
  • 最后修改日期: 2022-09-12
  • 录用日期: 2022-09-05
  • 在线发布日期: 2022-09-06
  • 出版日期: 2022-10-25