The cooling crystallization kinetics of 3, 4-bis(3-nitrofurazan-4-yl)furoxan (DNTF) in V(acetic acid):V(water)=7:3 mixed solvent was investigated by a batch crystallizer. Based on the equation of population balance, the nucleation and growth rate equation of DNTF cooling crystallization were determined performing multiple linear regression of experimental data by the least squares method. Results show that the indexes of crystal growth rate and nucleation rate of crystal for supersaturated degree are 3.83 and 1.3, respectively, meaning that the nucleation and growth rates of crystal increase with increasing the supersaturated degree of the system, but the growth rate increases faster. The index of crystal nucleation rate for the suspension particle density is up to 2.14, it means that with increasing the suspension particle density, the crystal nucleation rate increases significantly but the growth rate does not change, which are not favorable to crystal growth.